NGS Technologies: Illumina

Slides describing the principle behind Illumina technology

Transcript

1. Illumina Illumina: Chemistry, Molecular Biology and Data (2018)
2. Requirements ‣ Reversible termination ‣ Differentiation of nucleotides ‣ Ability to manipulate on solid support
3. Preliminary studies
4. Nucleotides Bentley et al. 2008
5. Solid support Fedurco et al. 2006
6. How it works
7. The overall idea
19. Metzker et al. 2010
20. Extending to paired ends
22. Multiplexing
23. Detection system G,T A,C
24. How it does not work
25. Errors
26. Phasing and Pre-phasing
27. Illumina chastity filter
28. Sequence Specific Errors Nakamura et al. 2011
29. Schematic representation of the (a) inverted repeat and (b) enzyme preference for the SSE hypothetical mechanistic models. Nakamura K et al. Nucl. Acids Res. 2011;39:e90-e90 © The Author(s) 2011. Published by Oxford University Press.
30. A typical run Minoche et al. 2011
31. Coverage as function of GC% Minoche et al. 2011
32. Low quality bases and alignment Minoche et al. 2011
33. Context dependency Minoche et al. 2011
35. True seq PCR free