LCG20

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1. @lcolladotor lcolladotor.github.io lcolladotor.github.io/bioc_team_ds Studying the human prefrontal cortex transcriptome at different resolutions Leonardo Collado Torres, Investigator #LCG20 at LCG-UNAM 󰐏 August 31 2023 Slides available at speakerdeck.com/lcolladotor
2. Interesadxs → Usarixs → Desarrolladores ¿Cómo le hacemos para fomentar este paso en México y Latino América?
3. • 2017: ◦ idea en BioC2017 e inicio de la fundación de CDSB • 2018: ◦ primer taller ^_^, con instructores de Bioconductor: Martin Morgan & Benilton Carvalho • 2019: ◦ BioC2019: apoyo a solicitud de becas ◦ Taller con materiales adaptados de RStudio • 2020: ◦ regutools: primer paquete en Bioconductor ◦ Taller con RStudio & Bioconductor • 2021: ◦ primera vez con 2 talleres https://comunidadbioinfo.github.io/
5. https://doi.org/10.1093/bioinformatics/btaa575
6. Junta Directiva CDSB hasta 2019
7. comunidadbioinfo.github.io
8. @lcolladotor lcolladotor.github.io lcolladotor.github.io/bioc_team_ds Studying the human prefrontal cortex transcriptome at different resolutions Leonardo Collado Torres, Investigator #LCG20 at LCG-UNAM 󰐏 August 31 2023 Slides available at speakerdeck.com/lcolladotor
9. doi.org/10.1016/j.biopsych.2020.06.005 Michael Gandal @mikejg84 Transcriptomic Insight Into the Polygenic Mechanisms Underlying Psychiatric Disorders
10. Background: Human DLPFC 10 slideshare.net Louise A Huuki-Myers @lahuuki
11. Zoom in: base pair resolution Jeff Leek @jtleek Ph.D. advisor Andrew E Jaffe @andrewejaffe Ph.D. co-advisor
12. lcolladotor.github.io/talk/lcg2014/ #LCG10 fue organizado por Esperanza Martínez Romero et al.
13. Flexible expressed region analysis for RNA-seq with #derfinder doi.org/10.1093/nar/gkw852 Jeff Leek @jtleek Ph.D. advisor
14. Fetal Infant Child Teen Adult 50+ 6 / group, N = 36 Discovery data Postmortem Human Brain Samples Fetal Infant Child Teen Adult 50+ 6 / group, N = 36 Replication data Andrew E Jaffe @andrewejaffe Ph.D. co-advisor Developmental regulation of human cortex transcription and its clinical relevance at single base resolution doi.org/10.1038/nn.3898 github.com/leekgroup/libd_n36
15. doi.org/10.1038/nn.3898 Developmental regulation of human cortex transcription and its clinical relevance at single base resolution github.com/leekgroup/libd_n36
16. Zoom in: more data! Ben Langmead @BenLangmead Abhinav Nellore @nellore (GitHub) Christopher Wilks @chrisnwilks Shannon Ellis @Shannon_E_Ellis Kasper Daniel Hansen @KasperDHansen Andrew E Jaffe @andrewejaffe Ph.D. co-advisor + LIBD former boss Jeff Leek @jtleek Ph.D. advisor
17. doi.org/10.1038/543007a
18. expression data for ~70,000 human samples samples phenotypes ? GTEx N=9,962 TCGA N=11,284 SRA N=49,848 samples expression estimates gene exon junctions ERs Answer meaningful questions about human biology and expression slide adapted from Shannon Ellis Reproducible RNA-seq analysis using #recount2 + Improving the value of public RNA-seq expression data by phenotype prediction doi.org/10.1038/nbt.3838 doi.org/10.1093/nar/gky102
19. recount3: over 700,000 human and mouse RNA-seq samples #recount3: summaries and queries for large-scale RNA-seq expression and splicing Christopher Wilks @chrisnwilks research.libd.org/recount3-docs/ doi.org/10.1186/s13059-021-02533-6
20. Zoom in: snRNA-seq → deconvolution of bulk RNA-seq Matthew N Tran @mattntran Kristen R Maynard @kr_maynard Louise A Huuki-Myers @lahuuki Keri Martinowich @martinowk Stephanie C Hicks @stephaniehicks
21. What is Deconvolution? • Inferring the composition of different cell types in a bulk RNA-seq data Louise A Huuki-Myers @lahuuki
22. Interaction eQTLs with cell type proportions github.com/LieberInstitute/goesHyde_mdd_rnaseq/tree/master/eqtl/code
23. Reference Single Cell Data 23 deconvolution(Y, Z) = Proportion of Cell Types Louise A Huuki-Myers @lahuuki
24. 10x snRNA-seq Reference Data AMY DLPFC HPC NAc sACC Astro 1638 782 1170 1099 907 Endo 31 0 0 0 0 Macro 0 10 0 22 0 Micro 1168 388 1126 492 784 Mural 39 18 43 0 0 Oligo 6080 5455 5912 6134 4584 OPC 1459 572 838 669 911 Tcell 31 9 26 0 0 Excit 443 2388 623 0 4163 Inhib 3117 1580 366 11476 3974 @mattntran Matthew N Tran doi.org/10.1016/j.neuron.2021.09.001
25. Marker Finding 25 deconvolution(Y, Z) = Proportion of Cell Types Louise A Huuki-Myers @lahuuki
26. 1vAll Markers vs. Mean Ratio Markers 26 Louise A Huuki-Myers @lahuuki research.libd.org/DeconvoBuddies/
27. 1vAll Markers vs. Mean Ratio Markers 27 Louise A Huuki-Myers @lahuuki research.libd.org/DeconvoBuddies/
28. Methods 28 deconvolution(Y, Z) = Proportion of Cell Types Louise A Huuki-Myers @lahuuki
29. Which Method is the Most Accurate? • Benchmarking shows that different methods perform best on different data sets (Cobos et al, Nature Communications, 2020) • Benchmarking results from different papers on “real” data ◦ MuSiC paper: MuSiC > NNLS > BSEQ-sx > CIBERSORT ▪ Pancreatic Islet: Beta cells vs. HbA1c (Fig 2a) ◦ Bisque paper: Bisque > MuSiC > CIBERSORT ▪ DLPFC: Microglia vs. Braak stage, Neuron vs. Cognitive diagnostic category (Fig 4) ◦ SCDC paper: SCDC > MuSiC > Bisque > DWLS > CIBERSORT ▪ Pancreatic Islet: Beta cells vs. HbA1c (Fig 4b) ◦ Cobos benchmark: DWLS > MuSiC > Bisque > deconvoSeq ▪ Human PMBC flow sorted (Fig 7) 29 Louise A Huuki-Myers @lahuuki
30. Results + Validation 30 deconvolution(Y, Z) = Proportion of Cell Types Louise A Huuki-Myers @lahuuki
31. Mean Proportions By Region: Tran et al, bioRxiv, 2020 (5 donors, 6 cell types) Louise A Huuki-Myers @lahuuki
32. Peric = Mural + Endo Mean Proportions By Region: Tran et al, Neuron, 2021 (8 donors, 10 cell types) Louise A Huuki-Myers @lahuuki
33. Sean Maden @MadenSean Sang Ho Kwon @sanghokwon17 #deconvochallenge doi.org/10.48550/arXiv.2305.06501
34. #deconvochallenge Challenges and opportunities to computationally deconvolve heterogeneous tissue with varying cell sizes using single cell RNA-sequencing datasets doi.org/10.48550/arXiv.2305.06501 Sean Maden @MadenSean
35. Sean Maden @MadenSean Sang Ho Kwon @sanghokwon17 #deconvochallenge doi.org/10.48550/arXiv.2305.06501
36. research.libd.org/TREG/ doi.org/10.1101/2022.04.28.489923 Louise A Huuki-Myers @lahuuki
37. Zoom in: spatial omics Kristen R Maynard @kr_maynard Keri Martinowich @martinowk Stephanie C Hicks @stephaniehicks Andrew E Jaffe @andrewejaffe Stephanie C Page @CerceoPage
38. Visium Platform for Spatial Gene Expression Image from 10x Genomics - A slide contains 4 capture areas, each full of thousands of 55um-wide “spots” (often containing 1-10 cells) - Unique barcodes in each spot bind to particular genes; after sequencing, gene expression can be tied back to exact spots, forming a spatial map Kristen R. Maynard 38
39. 2 pairs spatial adjacent replicates x subject = 12 sections 39 Subject 1 Subject 2 Subject 3 Adjacent spatial replicates (0μm) Adjacent spatial replicates (300μm) PCP4 Maynard, Collado-Torres, et al, Nat Neuro, 2021
40. “Pseudo-bulking” collapses data: spot to layer level 40 Maynard, Collado-Torres, et al, Nat Neuro, 2021
41. bioconductor.org/packages/spatialLIBD Pardo et al, 2022 DOI 10.1186/s12864-022-08601-w Maynard, Collado-Torres, 2021 DOI 10.1038/s41593-020-00787-0 Brenda Pardo Abby Spangler @PardoBree @abspangler Louise A. Huuki-Myers @lahuuki
42. DOI: 10.1038/s41593-020-00787-0 twitter.com/lcolladotor/status/1233661576433061888 from 2020-02-29 Andrew E Jaffe @andrewejaffe Kristen R Maynard @kr_maynard Keri Martinowich @martinowk
43. DOI: 10.1038/s41593-020-00787-0 twitter.com/lcolladotor/status/1233661576433061888 from 2020-02-29 DOI 10.1093/bioinformatics/btac299 Since Feb 2020 spatialLIBD::fetch_data() provides access to SpatialExperiment R/Bioconductor objects Stephanie C Hicks @stephaniehicks Lukas M Weber @lmweber
44. DOI: 10.1038/s41593-020-00787-0 twitter.com/lcolladotor/status/1233661576433061888 from 2020-02-29 twitter.com/CrowellHL/status/1597579271945715717 DOI 10.1093/bioinformatics/btac299 Since Feb 2020 spatialLIBD::fetch_data() provides access to SpatialExperiment R/Bioconductor objects
45. #spatialDLPFC 45 doi.org/10.1101/2023.02.15.528722 Louise A Huuki-Myers @lahuuki Abby Spangler @abspangler Nicholas J Eagles @Nick-Eagles (GitHub)
46. Different Resolutions of BayesSpace Clustering k = number of clusters • k=2: separate white vs. grey matter • k=9: best reiterated histological layers • k=16: data-driven optimal k based on fast H+ statistic 46 More Clusters = More Complexity doi.org/10.1101/2023.02.15.528722
47. Spatial Registration Adds Anatomical Context • Validate detection of laminar structure • Correlate enrichment t-statistics for top marker genes of reference ◦ Cluster vs. manual annotation • Annotate with strongly associated histological layer 47 Sp k D d ~L doi.org/10.1101/2023.02.15.528722
48. Spatial Registration of Spatial Domains • Map SpDs to Maynard et al. manual annotated layers • Highlight most strongly associated histological layer to add biological context 48 doi.org/10.1101/2023.02.15.528722
49. Identify Layer Associated Neuron Populations 49 • Apply Spatial Registration with manual layers • 13 layer-level cell types ◦ Assign Excitatory Neurons histological layers ◦ Pool other cell type groups Kelsey D Montgomery
50. Layer-level Cell Type Marker genes 50 Louise A Huuki-Myers @lahuuki
51. Spot Deconvolution 51 Cell 1 Cell 2 … Cell N Gene 1 0 0 … 0 Gene 2 2 5 … 3 … … … … … Gene i 1 0 … 0 Spot 1 Spot 2 … Spot M Gene 1 1 0 … 3 Gene 2 0 1 … 0 … … … … … Gene j 4 2 … 2 Astro Excit … Inhib Spot 1 1 1 … 1 Spot 2 … … … … … … Spot M 1 0 … 2 Single- Nucleus Spatial Deconvolved Results Spot 1 Nicholas J Eagles @Nick-Eagles (GitHub)
52. Existing Spot Deconvolution Software - Explored 3 novel software methods from the literature Software name Overall approach Input Cell Counts Output Tangram (Biancalani et al.) Mapping individual cells Every spot Integer counts Cell2location (Kleshchevnikov et al.) Matching gene-expression profile Average across spots Decimal counts SPOTlight (Elosua-Bayes et al.) Matching gene-expression profile Not used Proportions 52 Excit L5 Counts
53. Benchmarking Spot Deconvolution Software: Theory - How do we measure performance or accuracy of cell-type predictions? - Make orthogonal measurements*: image-derived counts - Leverage prior knowledge: neurons localize to gray matter? - Self-consistency of results: broad vs. fine cell-type results 53 Nicholas J Eagles @Nick-Eagles (GitHub) doi.org/10.1101/2023.02.15.528722
54. Visium Spatial Proteogenomics (SPG) Images as an Orthogonal Measurement 54 Nicholas J Eagles @Nick-Eagles (GitHub)
55. Visium Spatial Proteogenomics (Visium-SPG) Visium-SPG = Visium SRT + immunofluorescence (using identical tissue samples) Sang Ho Kwon @sanghokwon17
56. Visium Spatial Proteogenomics (Visium-SPG) - Gene expression captured like ordinary Visium - Multi-channel fluorescent images captured of the same tissue - Channels measure proteins marking for specific cell types Kristen R. Maynard 56 Sang Ho Kwon Visium-SPG = Visium SRT + immunofluorescence (using identical tissue samples) Fluorescent Protein Cell Type TMEM119 Microglia Neun Neurons OLIG2 Oligodendrocytes GFAP Astrocytes
57. 57 Max across layers Not max Benchmark Results: Leverage Prior Knowledge
58. Benchmark Results: Leverage Prior Knowledge 58
59. Benchmark Summary 59 Metric Tangram Cell2location SPOTlight Metric Type Avg. cor (spot-level) 0.31 0.30 0.21 Orthogonal measurements Avg. RMSE (spot-level) 1.35 1.24 1.3 Orthogonal measurements Overall prop.: (KL Div.) 0.44 0.49 0.41 Orthogonal measurements Overall prop.: (cor.) 0.46 0.37 0.47 Orthogonal measurements Overall prop.: (RMSE) 3020 3890 3040 Orthogonal measurements Histological mapping 0.69 0.77 0.23 Leverage known biology Broad vs. layer (cor.) 1.00 0.77 -0.36 Self-consistency of results Broad vs. layer (RMSE) 102 4200 4220 Self-consistency of results
60. Viewing Spot Deconvolution Results: Samui Browser - View: - Fluorescence channels - Spot deconvo results - Segmented cells - Gene expression - Interactive - Quickly zoom/scroll - Full-resolution images samuibrowser.com/from?url=data2.loopybrowser.com/VisiumIF/&s=Br2720_Ant_IF&s=Br6432_Ant_IF&s=Br6522_Ant_IF&s=Br8667_Post_IF Sriworarat, 2023. 60
61. Viewing Spot Deconvolution Results: spatialLIBD apps - View: - spot deconvolution results - spatial domains/ clusters - gene expression - Huge amount of aesthetic customization 61 https://libd.shinyapps.io/spatialDLPFC_Visium_SPG/
62. How Spot Deconvolution Results Were Used A. Better characterize unsupervised spatial domains B. Cell-cell communication; cell-type-informed ligand-receptor interactions in the context of schizophrenia risk A 62 Boyi Guo Melissa Grant-Peters
63. Visium spatial clustering works for variables with high % variance explained. But what about other ones? DOI: 10.1038/s41593-020-00787-0
64. twitter.com/sanghokwon17/status/1650589385379962881 from 2023-04-24 Sang Ho Kwon @sanghokwon17 DOI: 10.1101/2023.04.20.537710 #Visium_SPG_AD
65. Experimental design & study overview Braak V-VI & CERAD frequent Sang Ho Kwon
66. AD pathology signal is too small to detect by spatially-resolved gene expression alone research.libd.org/Visium_SPG_AD/
67. Identifying transcriptional signatures of AD-related neuropathology Sang Ho Kwon
68. Some challenges ⚠ with Visium 68
69. sc/snRNA-seq QC metrics such as # detected genes, # UMI, mitochondria expression % are likely biologically related!
70. Prashanthi Ravichandran @prashanthi-ravichandran (GH) Artifacts in general are normalized away by library size, though there are caveats
71. Diffusion issues: could be related to permeabilization step
72. Having more data is useful to provide context! Here 4 new samples have low sequencing saturation (outliers) but are within range of good samples from other studies
73. Having more data is useful to provide context! Those 4 samples have great median UMI counts per spot ^_^
74. Software keeps evolving and as leaders in the field we aim to use the best methods 74 Moses, L., Pachter, L. Museum of spatial transcriptomics. Nat Methods 19, 534–546 (2022). https://doi.org/10.1038/s41592-022-01409-2
75. The Development Process - Making a module - New, experimental software can change dramatically (function and syntax) between versions - Promotes collaboration by allowing two researchers to share exact code and instantly run software without special set-up SpatialExperiment release 3.14 SpatialExperiment devel 3.15 module load tangram/1.0.2 module load cell2location/0.8a0 module load spagcn/1.2.0 https://github.com/LieberInstitute/jhpce_mod_source https://github.com/LieberInstitute/jhpce_module_config Nicholas J Eagles @Nick-Eagles (GitHub)
76. The Development Process - Regular interaction with software authors to clarify functionality and report bugs - Documentation for code and author responsiveness on GitHub can be critical in successfully applying software to our data Nicholas J Eagles @Nick-Eagles (GitHub)
77. Documentation + wrapper functions + tests (GitHub Actions + Bioconductor) 77 bioconductor.org/packages/spatialLIBD
78. More challenges ahead Working with multiple capture areas per tissue Nicholas J Eagles @Nick-Eagles (GitHub) Prashanthi Ravichandran @prashanthi-ravichandran (GH) Spot diameter error: ~1.8 → ~1.1 Another pair: ~2.8 → ~0.76
79. lcolladotor.github.io/#projects • Every assay has caveats • We re-use tricks: think adding 0, multiplying by 1 • It nearly always takes a team • Data sharing accelerates science + democratizes access to it • Zooming in allows us to reduce the heterogeneity • We can learn from each other: from uniformly processing our data & re-using it → replicate / validate?
80. @MadhaviTippani Madhavi Tippani @HeenaDivecha Heena R Divecha @lmwebr Lukas M Weber @stephaniehicks Stephanie C Hicks @abspangler Abby Spangler @martinowk Keri Martinowich @CerceoPage Stephanie C Page @kr_maynard Kristen R Maynard @lcolladotor Leonardo Collado-Torres @Nick-Eagles (GH) Nicholas J Eagles Kelsey D Montgomery Sang Ho Kwon Image Analysis Expression Analysis Data Generation Thomas M Hyde @lahuuki Louise A Huuki-Myers @BoyiGuo Boyi Guo @mattntran Matthew N Tran @sowmyapartybun Sowmya Parthiban Slides available at speakerdeck.com /lcolladotor + Many more LIBD, JHU, and external collaborators @mgrantpeters Melissa Grant-Peters @prashanthi-ravichandran (GH) Prashanthi Ravichandran
81. lcolladotor.github.io @lcolladotor