sequencing has driven the development of high-throughput sequencing (or next-generation sequencing) technologies that parallelize the sequencing process, producing thousands or millions of sequences concurrently” from http://en.wikipedia.org/wiki/Next- generation_sequencing#Next-generation_methods! ! • High-throughput Sequencing, Massively Parallel Sequencing..! • ैདྷ๏ͱͷൺֱͰ͋Γ୯ҰͷγʔέϯεݪཧΛࢦ͢ͷͰͳ͍! • ୈnੈͱ͍͏ݺͼํΛ͢Δਓ͍Δ
single organism”! • ৽نήϊϜ! • ήϊϜͷಡ·Ε͍ͯͳ͍ੜͷήϊϜΛθϩ͔ΒNGSͰߏங͢Δ! • Assembleܕ! • Resequencing! • “Sequencing of a sample with respect to a reference”! • ήϊϜͷಡ·Εͨੜʹ͍ͭͯଟܕղੳൺֱήϊϜղੳΛߦ͏! • Mappingܕ
• ChIP-Seq! • “Direct sequencing of chromatin immunoprecipitates”! • Bisulfite-Seq! • “Sequencing following treatment of DNA with bisulfite to convert cytosine residues to uracil depending on methylation status"! • DNase-Seq! • “Sequencing of hypersensitive sites, or segments of open chromatin that are more readily cleaved by DNaseI."! • FAIRE-Seq! • “Formaldehyde-Assisted Isolation of Regulatory Elements"! • etc, etc..! • શͯϦϑΝϨϯεήϊϜͷmappingͰఆྔΛߦ͏
#SRAID 3059 204 id count title SRA008679 SRA030426 SRA024198 SRA008091 SRA000271 50 HapMap project 48 Human Prostate Cancer using Next Generation RNA Sequencing (human) 8 Metagenomic analysis of marine microbes isolated during the Global Ocean Sampling Expedition 8 Human 1000 genomes 7 HapMap project