the vicinity of the PB • PB domain sequesters mainly PIP2 lipids, but not PA or PS • PB diffusion rate weakly depends on undisturbed membrane composition • In the presence of lipid gradient PB drifts in the region of higher lipid density – spontaneous clustering
protein membrane-cytoplasmic shuttling – local lipid production – protein phosphorylation/dephosphorylation 2) Modelling of endocytic triggering and feedback loops