WT (from 0 min to 300 min) • p-value < 0.01 • 249 of genes affected by both mutations are also affected by EGF in WT PI3K+ & PTEN−/− EGF (WT) 4964 225 249
System development Anatomical structure morphogenesis Organ development Cardiovascular system development Blood vessel morphogenesis Angiogenesis 97 23 89 70 26 95 60 Locomotion Chemotaxis Neutrophil chemotaxis Leucocyte chemotaxis Immune system process Cell adhesion Response to external stimulus Response to stress Response to wounding 30 33 40 33 21 6 GO enrichment analysis Genes Genes
Genes Genes PIK3IP1! negative regulator of hepatic PI3K activity! GAB2! involved in the activation of PI3K! DENND2D! GEF, tumour suppressor in lung cancer, PI3K sensitive! LPXN! interacts with tyrosine kinases ! (upstream of PI3K)! VWA5A! breast cancer suppressor! TACC2! tumor suppressor
Pnar Pir, Martina Froehlich, Nicolas Rodriguez, Massimo Lai, Lu Li • Stephens/Hawkins labs: Len Stephens, Phil Hawkins, Veronique Juvin, Mouhannad Malek • Luscombe lab: Nicholas Luscombe, Kathi Zarnack, Raphaelle Luisier, Filipe Tavares-Cadete, Bori Mifsud, Anna Poetsch, Alessandra Vigilante, Elodie Darbo, Robert Sugar, Inigo Martincorena, Juanma Vaquerizas, Judith Zaugg • BI bioinformatics group: Simon Andrews, Anne Segonds-Pichon, Phil Ewels, Felix Krueger • Others: Sven Bergmann, Mikhail Spivakov